It seems, and this is hardly a scientific or tested answer, that the Profiles, being cloned on the same page is picking up the number of profiles per row from the first instance and not allowing the second, cloned, instance from using any settings you're applying there.
I'm going to report this to our developers.
Meanwhile, to help you affix a suitable solution, I'm going to recommend you use a different option. Of course, the choice is yours, but I'd replace our Profiles with
That plugin uses something called "Content Blocks" which are essentially the same as boxes, but have far more capabilities, such as order setting and distinct controls over layout. The best part is, you can apply them to a page by shortcode. Each shortcode calls a particular set, as with boxes, so you can place two people in one set, three in another, and one in the last if you like. You may also apply CSS to each individual content block, to achieve the same effect Profiles provides.
I use it on the front page of my own site and while it takes a bit of time to learn, once you do, the plugin is great. Additionally, unlike many plugins out there, this is very professionally maintained and kept up to date by its developer. There's also an inexpensive Pro edition at codecanyon.net.